A key role of nuclear factor Y in the refeeding response of fatty acid synthase in adipocytes

FEBS Lett. 2017 Apr;591(7):965-978. doi: 10.1002/1873-3468.12620. Epub 2017 Mar 27.

Abstract

Fatty acid synthase (Fasn) is a key component of energy metabolism that is dynamically induced by food intake. Although extensive studies have revealed a number of transcription factors involved in the fasting/refeeding transition of Fasn expression in hepatocytes, much less evidence is available for adipocytes. Using the in vivo Ad-luc analytical system, we identified the inverted CCAAT element (ICE) around -100 nucleotides in the Fasn promoter as a critical cis-element for the refeeding response in adipocytes. Electrophoretic mobility shift assays and chromatin immunoprecipitation show that nuclear factor Y (NF-Y) binds to ICE specifically in refeeding states. Notably, the NF-Y binding to ICE is differently regulated between adipocytes and hepatocytes. These findings provide insights into the specific mechanisms controlling energy metabolism in adipocytes.

Keywords: adipose tissue; fatty acid; transcription.

MeSH terms

  • 3T3-L1 Cells
  • Adenoviridae / genetics
  • Adipocytes / cytology
  • Adipocytes / metabolism*
  • Adipose Tissue, White / metabolism
  • Animals
  • Base Sequence
  • CCAAT-Binding Factor / genetics
  • CCAAT-Binding Factor / metabolism*
  • Chromatin Immunoprecipitation
  • Electrophoretic Mobility Shift Assay
  • Fatty Acid Synthases / genetics
  • Fatty Acid Synthases / metabolism*
  • Feeding Behavior*
  • Gene Expression Regulation
  • Immunoblotting
  • Liver / metabolism
  • Luciferases / genetics
  • Luciferases / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred ICR
  • Mutation
  • Promoter Regions, Genetic / genetics
  • Protein Binding
  • Response Elements / genetics
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • CCAAT-Binding Factor
  • nuclear factor Y
  • Luciferases
  • Fatty Acid Synthases