Elemental ion release and cytotoxicity of antimicrobial acrylic resins incorporated with nanomaterial

Objectives: This study evaluated the release of ions and the cytotoxicity of acrylic resins incorporated with silver vanadate decorated with silver nanoparticles (AgVO₃ ).

Background: The inhibition of the accumulation of microorganisms on the resins is critical in preventing diseases. However, the hypothesis is that the release of ions from the incorporation of AgVO₃ may be important in biocompatibility.

Materials and methods: Specimens of autopolymerising (AP) and heat-polymerising resin (HP) with AgVO₃ were prepared and immersed in culture medium. The release of silver ions (Ag) and vanadium (V) was evaluated by mass spectrometry with inductively coupled plasma (ICP-MS) (n=9) and the cell viability of fibroblasts L929 by MTT (3-[4,5-dimethylthiazol- 2yl]-2,5-diphenyltetrazolium bromide) (n=12). The results were evaluated with analysis of variance (ANOVA), Tukey and Pearson correlation test (α=.05).

Results: The groups containing AgVO₃ presented a difference in relation to the control (0%) regarding the release of Ag and V (P<.0001). All groups showed a reduction in L929 viability when compared with the cellular control (100%) (P<.0001). In comparison with the control resins for HP, a reduction in the metabolism of cells occurred starting at 2.5% and for AP at 5% (P<.0001). A positive correlation was found between the concentration of AgVO₃ and the ion release, and a negative between the ion release and the cell viability.

Conclusions: Significant numbers of Ag and V ions were released from resins with higher concentrations of AgVO₃ , presenting cytotoxicity for cells, suggesting that the use of low concentrations is indicated to avoid risks to patients.