Format

Send to

Choose Destination
J Dermatol Sci. 2017 Aug;87(2):168-175. doi: 10.1016/j.jdermsci.2017.04.014. Epub 2017 Apr 30.

High-glucose environment induced intracellular O-GlcNAc glycosylation and reduced galectin-7 expression in keratinocytes: Implications on impaired diabetic wound healing.

Author information

1
Department of Dermatology, Kaohsiung Medical University Hospital, Department of Dermatology, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
2
Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung, Taiwan.
3
Department of Dermatology, Kaohsiung Medical University Hospital, Department of Dermatology, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; Center for Biomarkers and Biotech Drugs, Kaohsiung Medical University, Kaohsiung, Taiwan. Electronic address: laneric@cc.kmu.edu.tw.

Abstract

BACKGROUND:

Diabetes is an important global health issue due to its increasing prevalence and association with various complications. Impaired wound healing is a serious complication associated with diabetes that frequently results in infection and amputation. Galectin-7 (Gal-7) has been reported to play an important role during skin wound healing. Previously, we had demonstrated that high glucose environment alters physiologic functions of keratinocytes and contributes to impaired wound healing in diabetic condition.

OBJECTIVE:

In this study, we hypothesized that Gal-7 expression of keratinocytes may be involved in delayed wound healing of diabetics.

METHODS:

Using cultured human keratinocytes and diabetic mice model, the Gal-7 expression was evaluated under high glucose environment.

RESULTS:

Our results demonstrated that high-glucose environment reduced Gal-7 expression, a molecule that plays an important role in keratinocyte migration. Additionally, we found that increased O-linked N-Acetyl-glucosamine (O-GlcNAc) is responsible for reduced Gal-7 expression in keratinocytes exposed to high glucose environment.

CONCLUSION:

Taken together, restoring the levels of Gal-7 and O-GlcNAc glycosylation may present novel therapeutic approach to promote wound healing in diabetic patients.

KEYWORDS:

Galectin-7; Glucose; Healing; Keratinocytes; O-GlcNAc glycosylation

PMID:
28526214
DOI:
10.1016/j.jdermsci.2017.04.014
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center