Resveratrol induces membrane and DNA disruption via pro-oxidant activity against Salmonella typhimurium

Biochem Biophys Res Commun. 2017 Jul 22;489(2):228-234. doi: 10.1016/j.bbrc.2017.05.138. Epub 2017 May 25.

Abstract

Resveratrol is a flavonoid found in various plants including grapes, which has been reported to be active against various pathogenic bacteria. However, antibacterial effects and mechanisms via pro-oxidant property of resveratrol remain unknown and speculative. This research investigated antibacterial mechanism of resveratrol against a food-borne human pathogen Salmonella typhimurium, and confirmed the cell death associated oxidative damage. Resveratrol increased outer membrane permeability and membrane depolarization. It also was observed DNA injury responses such as DNA fragmentation, increasing DNA contents and cell division inhibition. Intracellular ROS accumulation, GSH depletion and significant increased malondialdehyde levels were confirmed, which indicated pro-oxidant activity of resveratrol and oxidative stress. Furthermore, the observed lethal damages were reduced by antioxidant N-acetylcysteine treatment supported the view that resveratrol-induced oxidative stress stimulated S. typhimurium cell death. In conclusion, this study expands understanding on role of pro-oxidant property and insight into previously unrecognized oxygen-dependent anti-Salmonella mechanism on resveratrol.

Keywords: Antibacterial activity; Oxidative stress; Prooxidant; Resveratrol; Salmonella typhimurium.

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Cell Membrane / drug effects*
  • Cell Membrane / metabolism
  • Cell Survival / drug effects
  • DNA, Bacterial / drug effects*
  • DNA, Bacterial / metabolism
  • Microbial Sensitivity Tests
  • Oxidants / pharmacology*
  • Oxidation-Reduction / drug effects
  • Oxidative Stress / drug effects
  • Resveratrol
  • Salmonella typhimurium / cytology
  • Salmonella typhimurium / drug effects*
  • Salmonella typhimurium / metabolism
  • Stilbenes / pharmacology*
  • Structure-Activity Relationship

Substances

  • Anti-Bacterial Agents
  • DNA, Bacterial
  • Oxidants
  • Stilbenes
  • Resveratrol