Long non-coding RNA PCAT7 regulates ELF2 signaling through inhibition of miR-134-5p in nasopharyngeal carcinoma

Biochem Biophys Res Commun. 2017 Sep 16;491(2):374-381. doi: 10.1016/j.bbrc.2017.07.093. Epub 2017 Jul 17.

Abstract

While some long noncoding RNAs (lncRNAs) might promote nasopharyngeal carcinoma (NPC) initiation and progression, the involved molecular mechanisms remain largely unclear. Here, we discovered the novel LncRNA, prostate cancer associated transcript 7 (PCAT7), which was overexpressed and associated with worse prognosis in NPC. Decreased PCAT7 expression was found to significantly suppress tumor cell proliferation in vitro, and inhibited tumor growth and reduced the expression of proliferation antigen Ki-67 in vivo. Rescue assay was performed to further confirm that PCAT7 contributed to the progression of NPC through regulating miR-134-5p/ELF2 signal pathway. These results indicated that PCAT7 might contribute to the tumor progression in NPC by functioning as a ceRNA to sponge miR-134-5p.

Keywords: ELF2; Nasopharyngeal carcinoma (NPC); Prostate cancer associated transcript 7 (PCAT7); Tumorigenesis; miR-134-5p.

MeSH terms

  • Base Sequence
  • Binding Sites
  • Carcinogenesis
  • Carcinoma / diagnosis*
  • Carcinoma / genetics*
  • Carcinoma / mortality
  • Carcinoma / pathology
  • Cell Line, Tumor
  • Cell Proliferation
  • Cell Survival
  • Gene Expression Regulation, Neoplastic*
  • Genes, Reporter
  • Humans
  • Ki-67 Antigen / genetics
  • Ki-67 Antigen / metabolism
  • Luciferases / genetics
  • Luciferases / metabolism
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Nasopharyngeal Carcinoma
  • Nasopharyngeal Neoplasms / diagnosis*
  • Nasopharyngeal Neoplasms / genetics*
  • Nasopharyngeal Neoplasms / mortality
  • Nasopharyngeal Neoplasms / pathology
  • Prognosis
  • RNA, Long Noncoding / genetics*
  • RNA, Long Noncoding / metabolism
  • Signal Transduction
  • Survival Analysis
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism

Substances

  • Ki-67 Antigen
  • MIRN134 microRNA, human
  • MicroRNAs
  • RNA, Long Noncoding
  • Transcription Factors
  • long non-coding RNA PCAT7, human
  • ELF2 protein, human
  • Luciferases