Molecular characterization and antimicrobial resistance profile of atypical Citrobacter gillenii and Citrobacter sp. isolated from diseased rainbow trout (Oncorhynchus mykiss)

Muhammed Duman; Izzet Burcin Saticioglu; Ayse Gul Buyukekiz; Fikri Balta; Soner Altun

doi:10.1016/j.jgar.2017.05.014

Molecular characterization and antimicrobial resistance profile of atypical Citrobacter gillenii and Citrobacter sp. isolated from diseased rainbow trout (Oncorhynchus mykiss)

J Glob Antimicrob Resist. 2017 Sep:10:136-142. doi: 10.1016/j.jgar.2017.05.014. Epub 2017 Jul 18.

Authors

Muhammed Duman¹, Izzet Burcin Saticioglu¹, Ayse Gul Buyukekiz¹, Fikri Balta², Soner Altun³

Affiliations

¹ Uludag University, Faculty of Veterinary Medicine, Aquatic Animal Disease Department, Turkey.
² Recep Tayyip Erdogan University, Faculty of Fisheries and Aquatic Sciences, Department of Aquaculture, Turkey.
³ Uludag University, Faculty of Veterinary Medicine, Aquatic Animal Disease Department, Turkey. Electronic address: saltun@uludag.edu.tr.

PMID: 28732787
DOI: 10.1016/j.jgar.2017.05.014

Abstract

Aim: Over the last decade, Citrobacter species have been responsible for infections in fish and many species and also new Citrobacter species have been identified. In this study, molecular identifications and the phenotypic and genotypic antimicrobial-resistance characteristics of atypical and typical Citrobacter species were determined.

Materials and methods: Seven Citrobacter isolates were investigated from rainbow trout of different lengths with signs of disease. Biochemical characteristics were determined using conventional tests and rapid test kits; moreover, molecular identifications were conducted with 16S rRNA and the gyrB housekeeping gene region. The sequencing results obtained from the gyrB gene region were deposited in the GenBank database and compared with isolates from different countries that were registered in the database. Resistances to florfenicol, sulfonamides, and tetracycline antimicrobials were determined using the broth micro dilution method, and molecular resistance genes against these antimicrobials were identified. All detected resistance genes were confirmed by sequence analyses.

Results: It was determined that three of the Citrobacter species with biochemical characteristics were atypical and showed oxidase-positive reactions. All the Citrobacter species were identified as Citrobacter sp. using the 16S rRNA gene; three isolates were identified as Citrobacter gillenii and four as Citrobacter sp. based on gyrB gene sequence analysis. Some isolates were found in the same group as other countries' isolates in the GenBank database, while isolates with high identities were found in different genogroups. All isolates were found to be phenotypically resistant to sulfamethoxazole and susceptible to tetracycline; these isolates' resistance genes included sulI, tetA, tetB, and tetD.

Keywords: Antimicrobial resistance genes; Atypical Citrobacter; Citrobacter gillenii; Molecular characterization.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anti-Bacterial Agents / pharmacology
Aquaculture
Citrobacter / drug effects
Citrobacter / genetics*
Citrobacter / isolation & purification*
Citrobacter / pathogenicity
DNA Gyrase / genetics
DNA, Bacterial
Drug Resistance, Bacterial / genetics*
Fish Diseases / microbiology
Genes, Bacterial / genetics
Genes, Essential / genetics
Genotype*
Microbial Sensitivity Tests
Molecular Typing
Oncorhynchus mykiss / microbiology*
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA

Substances

Anti-Bacterial Agents
DNA, Bacterial
RNA, Ribosomal, 16S
DNA Gyrase