Plasma virome of cattle from forest region revealed diverse small circular ssDNA viral genomes

Hao Wang; Shouxin Li; Asif Mahmood; Shixing Yang; Xiaochun Wang; Quan Shen; Tongling Shan; Xutao Deng; Jingjiao Li; Xiuguo Hua; Li Cui; Eric Delwart; Wen Zhang

doi:10.1186/s12985-018-0923-9

Plasma virome of cattle from forest region revealed diverse small circular ssDNA viral genomes

Virol J. 2018 Jan 15;15(1):11. doi: 10.1186/s12985-018-0923-9.

Authors

Hao Wang^{1

2}, Shouxin Li^{3

4}, Asif Mahmood¹, Shixing Yang¹, Xiaochun Wang¹, Quan Shen¹, Tongling Shan⁵, Xutao Deng⁶, Jingjiao Li⁷, Xiuguo Hua⁷, Li Cui⁷, Eric Delwart⁶, Wen Zhang⁸

Affiliations

¹ Department of Microbiology, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212013, China.
² Department of Swine Infectious Disease, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China.
³ College of Wildlife Resources, Northeast Forestry University, Harbin, Heilongjiang, 150040, China.
⁴ College of Life Science, Shenyang Normal University, Shenyang, Liaoning, 110034, China.
⁵ Department of Swine Infectious Disease, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China. shantongling@shvri.ac.cn.
⁶ Blood Systems Research Institute, Department of Laboratory Medicine, University of California San Francisco, San Francisco, CA, 94118, USA.
⁷ School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, 200240, China.
⁸ Department of Microbiology, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212013, China. z0216wen@yahoo.com.

Abstract

Background: Free-range cattle are common in the Northeast China area, which have close contact with farmers and may carry virus threatening to cattle and farmers.

Methods: Using viral metagenomics we analyzed the virome in plasma samples collected from 80 cattle from the forested region of Northeast China.

Results: The virome of cattle plasma is composed of the viruses belonging to the families including Parvoviridae, Papillomaviridae, Picobirnaviridae, and divergent viral genomes showing sequence similarity to circular Rep-encoding single stranded (CRESS) DNA viruses. Five such CRESS-DNA genomes were full characterized, with Rep sequences related to circovirus and gemycircularvirus. Three bovine parvoviruses belonging to two different genera were also characterized.

Conclusion: The virome in plasma samples of cattle from the forested region of Northeast China was revealed, which further characterized the diversity of viruses in cattle plasma.

Keywords: CRESS-DNA virus; Cattle blood; Parvovirus; Virome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Biodiversity
Cattle
Cattle Diseases / virology*
DNA Viruses / genetics*
DNA, Circular*
DNA, Viral*
Forests
Genetic Variation*
Genome, Viral
High-Throughput Nucleotide Sequencing
Metagenome
Metagenomics / methods
Phylogeny
Sequence Analysis, DNA
Viral Load*
Virus Diseases / veterinary*

Substances

DNA, Circular
DNA, Viral