Genetic Diversity among Staphylococcus aureus Isolates Showing Oxacillin and/or Cefoxitin Resistance Not Linked to the Presence of mec Genes

Antimicrob Agents Chemother. 2018 Jun 26;62(7):e00091-18. doi: 10.1128/AAC.00091-18. Print 2018 Jul.

Abstract

Methicillin-resistant Staphylococcus aureus isolates lacking mec genes (n = 32), collected from Belgian hospitals, were characterized for their β-lactamase production and the presence of mutations in pbp genes, the pbp4 promoter, and genes involved in penicillin-binding protein 4 overproduction (gdpP and yjbH). Twelve isolates were β-lactamase hyperproducers (BHPs), while 12 non-BHP isolates might produce an incomplete GdpP protein. Most isolates showed nucleotide missense mutations in pbp genes. A few isolates also showed mutations in the pbp4 promoter.

Keywords: BORSA; MODSA; PBP; β-lactamase.

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Bacterial Proteins / genetics*
  • Cefoxitin / pharmacology*
  • Humans
  • Methicillin-Resistant Staphylococcus aureus / drug effects*
  • Methicillin-Resistant Staphylococcus aureus / genetics*
  • Methicillin-Resistant Staphylococcus aureus / isolation & purification
  • Microbial Sensitivity Tests
  • Oxacillin / pharmacology*
  • Penicillin-Binding Proteins / genetics*
  • Retrospective Studies
  • Staphylococcal Infections / drug therapy
  • Staphylococcal Infections / microbiology
  • beta-Lactam Resistance / genetics*
  • beta-Lactamases / genetics

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Penicillin-Binding Proteins
  • mecA protein, Staphylococcus aureus
  • Cefoxitin
  • beta-Lactamases
  • Oxacillin