The interferon-inducible protein p202 promotes osteogenesis in mouse bone marrow stromal cells

Biosci Rep. 2018 Jun 27;38(3):BSR20171618. doi: 10.1042/BSR20171618. Print 2018 Jun 29.

Abstract

In the present study, we explored the role of the interferon-inducible protein p202 in osteoblast differentiation of mouse bone marrow stromal cells (BMSCs). Both the mRNA and protein levels of p202 increased initially and decreased afterward in the course of BMSC osteogenesis. The intracellular distribution of this protein also changed in the differentiation process. p202 knockdown inhibited, while p202 overexpression enhanced, the osteoblast differentiation of BMSCs. This was identified by evaluation of expression of osteogenic markers, Alizarin Red S staining, and determination of alkaline phosphatase activity. Further study revealed that p202 disturbs the formation of Runx2/Ids complex and frees Runx2 to induce the differentiation process. The findings demonstrated that p202 plays a positive role in BMSC osteogenesis.

Keywords: Bone marrow stromal cells (BMSCs); Inhibitor of differentiation (Id) proteins; Interferon-inducible protein p202; Osteoblast differentiation; Runx2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaline Phosphatase / genetics
  • Alkaline Phosphatase / metabolism
  • Animals
  • Anthraquinones
  • Bone Marrow Cells / cytology
  • Bone Marrow Cells / metabolism*
  • Cell Differentiation
  • Core Binding Factor Alpha 1 Subunit / genetics
  • Core Binding Factor Alpha 1 Subunit / metabolism
  • Female
  • Femur / cytology
  • Femur / metabolism
  • Gene Expression Regulation
  • Intracellular Signaling Peptides and Proteins / antagonists & inhibitors
  • Intracellular Signaling Peptides and Proteins / genetics*
  • Intracellular Signaling Peptides and Proteins / metabolism
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / metabolism*
  • Mice
  • Osteoblasts / cytology
  • Osteoblasts / metabolism*
  • Osteogenesis / genetics*
  • Primary Cell Culture
  • RNA, Small Interfering / genetics
  • RNA, Small Interfering / metabolism
  • Signal Transduction
  • Tibia / cytology
  • Tibia / metabolism

Substances

  • Anthraquinones
  • Core Binding Factor Alpha 1 Subunit
  • Ifi202b protein, mouse
  • Intracellular Signaling Peptides and Proteins
  • RNA, Small Interfering
  • Runx2 protein, mouse
  • Alizarin Red S
  • Alkaline Phosphatase