The N-terminal polypeptide derived from vMIP-II exerts its anti-tumor activity in human breast cancer by regulating lncRNA SPRY4-IT1

Biosci Rep. 2018 Oct 17;38(5):BSR20180411. doi: 10.1042/BSR20180411. Print 2018 Oct 31.

Abstract

Accumulating evidence demonstrates that long non-coding RNA (lncRNA) sprouty4-intron transcript 1 (lncRNA SPRY4-IT1) plays a vital role in the development of breast cancer. However, the underlying mechanism has not been eventually illuminated. We aimed to explore the biological activity of lncRNA SPRY4-IT1 in breast cancer cells and whether N-terminal polypeptide derived from viral macrophage inflammatory protein II (NT21MP) could exert its anti-tumor effect by regulating lncRNA SPRY4-IT1 and its target gene SKA2 Real-time RT-PCR, Western blotting, wound healing, and invasion assays were used to achieve this goal. We found that lncRNA SPRY4-IT1 was highly expressed in breast cancer cells. Moreover, NT21MP markedly inhibited biological effects of breast cancer cells by regulating lncRNA SPRY4-IT1, which was partially achieved through SKA2. Our findings suggested that lncRNA SPRY4-IT1 could serve as a novel biomarker by NT21MP for breast cancer.

Keywords: Breast cancer; NT21MP; SKA2; SPRY4-IT1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers, Tumor / genetics
  • Breast Neoplasms / drug therapy*
  • Breast Neoplasms / genetics
  • Breast Neoplasms / pathology
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Chemokines / genetics
  • Chemokines / pharmacology*
  • Chromosomal Proteins, Non-Histone / genetics*
  • Epithelial-Mesenchymal Transition / genetics
  • Female
  • Gene Expression Regulation, Neoplastic / drug effects
  • Herpesviridae / chemistry
  • Herpesviridae / genetics
  • Humans
  • RNA, Long Noncoding / genetics*

Substances

  • Biomarkers, Tumor
  • Chemokines
  • Chromosomal Proteins, Non-Histone
  • RNA, Long Noncoding
  • SKA2 protein, human
  • long noncoding RNA SPRY4-IT1, human
  • vMIP-II