Adult forms of the Ca2+ATPase of sarcoplasmic reticulum. Expression in developing skeletal muscle

J Biol Chem. 1987 Mar 15;262(8):3768-74.

Abstract

Two separate genes encode fast-twitch and slow-twitch/cardiac muscle forms of the Ca2+ ATPase of sarcoplasmic reticulum. Full length Ca2+ ATPase clones have been isolated from adult rabbit fast-twitch, slow-twitch, and cardiac muscles. Segments of these clones containing unique sequences have been used as probes to study developmental changes in Ca2+ ATPase transcripts. The fast-twitch Ca2+ ATPase transcript undergoes developmentally regulated alternative splicing in which a penultimate 42-base pair exon is retained in the adult transcript but is excised in the neonatal transcript. This additional exon shifts the exon encoding the neonatal carboxyl-terminal sequence, -Asp-Pro-Glu-Asp-Glu-Arg-Arg-Lys (Brandl, C. J., Green, N. M., Korczak, B., and MacLennan, D. H. (1986) Cell 44, 597-607) into a nontranslated region and results in the expression of an adult isoform with a carboxyl-terminal -Gly. The neonatal form of the fast-twitch Ca2+ ATPase represents 72% of the fast-twitch Ca2+ ATPase transcripts just prior to birth but only 17% by 14 days of age and 4% in adult fast-twitch muscle. Adult slow-twitch, adult cardiac, and neonatal skeletal muscles express an identical Ca2+ATPase mRNA transcript which is distinct from either of the fast-twitch forms. The slow-twitch/cardiac Ca2+ ATPase is the predominant form expressed in late fetal and early neonatal rabbit skeletal muscle, but this form is lost as the skeletal muscle differentiates into a fast-twitch state. Three or more alternative polyadenylation signals exist for this mRNA in all tissues with the most 3' signal predominating.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Calcium-Transporting ATPases / genetics*
  • Cloning, Molecular
  • DNA / metabolism
  • DNA Restriction Enzymes
  • Heart / growth & development*
  • Isoenzymes / genetics*
  • Muscle Development*
  • Muscles / enzymology
  • Myocardium / enzymology
  • Rabbits
  • Sarcoplasmic Reticulum / enzymology*
  • Transcription, Genetic*

Substances

  • Isoenzymes
  • DNA
  • DNA Restriction Enzymes
  • Calcium-Transporting ATPases

Associated data

  • GENBANK/J02682
  • GENBANK/M15158
  • GENBANK/M15159
  • GENBANK/M15351
  • GENBANK/X02814