Synergistic effect of mixture of two proline-rich-protein salivary families (aPRP and bPRP) on the interaction with wine flavanols

A M Ramos-Pineda; I García-Estévez; S Soares; V de Freitas; M Dueñas; M T Escribano-Bailón

doi:10.1016/j.foodchem.2018.08.024

Synergistic effect of mixture of two proline-rich-protein salivary families (aPRP and bPRP) on the interaction with wine flavanols

Food Chem. 2019 Jan 30:272:210-215. doi: 10.1016/j.foodchem.2018.08.024. Epub 2018 Aug 8.

Authors

A M Ramos-Pineda¹, I García-Estévez², S Soares³, V de Freitas³, M Dueñas¹, M T Escribano-Bailón¹

Affiliations

¹ Grupo de Investigación en Polifenoles (GIP), Facultad de Farmacia, University of Salamanca, Salamanca, Spain.
² Grupo de Investigación en Polifenoles (GIP), Facultad de Farmacia, University of Salamanca, Salamanca, Spain. Electronic address: igarest@usal.es.
³ REQUIMTE/LAQV, Faculdade de Ciências da Universidade do Porto, Porto, Portugal.

PMID: 30309534
DOI: 10.1016/j.foodchem.2018.08.024

Abstract

In this study, we have evaluated by HPLC-DAD, DLS and MALDI-TOF a synergic effect of the coexistence of two salivary-PRP fractions (basic-PRPs and acidic PRPs) on the interaction with flavanols. Results obtained showed noticeable enhancement of the interaction between (epi)catechin and PRPs when both types of proteins are blended. Up to 30 soluble aggregates have been tentatively identified with molecular weight from 4680 to 35,851. (epi)Catechins seem to bind preferentially bPRPs than aPRPs, although the medium size aggregates flavanol-bPRPs formed could favour the interaction with aPRPs giving rise to soluble mixed aggregates.

Keywords: Astringency; Dynamic-light-scattering; Flavan-3-ols; HPLC-DAD; Interaction; MALDI-TOF; Salivary proline-rich proteins.

MeSH terms

Drug Synergism
Flavonoids / pharmacology*
Salivary Proline-Rich Proteins / chemistry
Salivary Proline-Rich Proteins / pharmacology*
Sensation / drug effects
Solubility
Wine / analysis*

Substances

Flavonoids
Salivary Proline-Rich Proteins