Mycobacterium talmoniae, a Potential Pulmonary Pathogen Isolated from Multiple Patients with Bronchiectasis in the United States, Including the First Case of Clinical Disease in a Patient with Cystic Fibrosis

Ravikiran Vasireddy; Sruthi Vasireddy; Barbara A Brown-Elliott; Alexander L Greninger; Rebecca M Davidson; Kevin L Ard; Christine Y Turenne; Richard J Wallace Jr

doi:10.1128/JCM.00906-18

Mycobacterium talmoniae, a Potential Pulmonary Pathogen Isolated from Multiple Patients with Bronchiectasis in the United States, Including the First Case of Clinical Disease in a Patient with Cystic Fibrosis

J Clin Microbiol. 2019 Jan 30;57(2):e00906-18. doi: 10.1128/JCM.00906-18. Print 2019 Feb.

Authors

Ravikiran Vasireddy^#¹, Sruthi Vasireddy^#¹, Barbara A Brown-Elliott², Alexander L Greninger³, Rebecca M Davidson⁴, Kevin L Ard⁵, Christine Y Turenne⁶, Richard J Wallace Jr¹

Affiliations

¹ Mycobacteria/Nocardia Research Laboratory, Department of Microbiology, The University of Texas Health Science Center at Tyler, Tyler, Texas, USA.
² Mycobacteria/Nocardia Research Laboratory, Department of Microbiology, The University of Texas Health Science Center at Tyler, Tyler, Texas, USA Barbara.Elliott@uthct.edu.
³ Department of Laboratory Medicine, University of Washington, Seattle, Washington, USA.
⁴ Center for Genes, Environment and Health, National Jewish Health, Denver, Colorado, USA.
⁵ Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA.
⁶ Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, Manitoba, Canada.

^# Contributed equally.

Abstract

We characterize three respiratory isolates of the recently described species Mycobacterium talmoniae recovered in Texas, Louisiana, and Massachusetts, including the first case of disease in a patient with underlying cystic fibrosis. The three isolates had a 100% match to M. talmoniae NE-TNMC-100812^T by complete 16S rRNA, rpoB region V, and hsp65 gene sequencing. Core genomic comparisons between one isolate and the type strain revealed an average nucleotide identity of 99.8%. The isolates were susceptible to clarithromycin, amikacin, and rifabutin, while resistance was observed for tetracyclines, ciprofloxacin, and linezolid. M. talmoniae should be added to the list of potential pulmonary pathogens, including in the setting of cystic fibrosis.

Keywords: Mycobacterium talmoniae; bronchiectasis; cystic fibrosis.

Publication types

Case Reports

MeSH terms

Aged
Anti-Bacterial Agents / pharmacology
Bacterial Proteins / genetics
Bronchiectasis / complications*
Chaperonin 60 / genetics
Child
Cluster Analysis
Cystic Fibrosis / complications*
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
DNA-Directed RNA Polymerases / genetics
Female
Humans
Louisiana
Lung Diseases, Fungal / diagnosis*
Lung Diseases, Fungal / microbiology
Male
Massachusetts
Microbial Sensitivity Tests
Mycobacterium / classification*
Mycobacterium / drug effects
Mycobacterium / genetics
Mycobacterium / isolation & purification*
Mycobacterium Infections / diagnosis*
Mycobacterium Infections / microbiology
Phylogeny*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Texas

Substances

Anti-Bacterial Agents
Bacterial Proteins
Chaperonin 60
DNA, Bacterial
DNA, Ribosomal
RNA, Ribosomal, 16S
heat-shock protein 65, Mycobacterium
DNA-Directed RNA Polymerases
RNA polymerase beta subunit

Supplementary concepts

Mycobacterium talmoniae