Whole-genome sequencing of an NDM-1- and OXA-58-producing Acinetobacter towneri isolate from hospital sewage in Sichuan Province, China

Objectives: Acinetobacter spp. isolates carrying the bla_NDM-1 gene are frequently reported. However, most reported bla_NDM-1 genes are carried by clinical strains. Here we report a carbapenem-resistant Acinetobacter towneri isolate from hospital sewage in China co-harbouring bla_NDM-1 and bla_OXA-58 in the genome.

Methods: Whole-genome sequencing was performed using a single molecule, real-time (SMRT) sequencing platform with a Pacific Biosciences RS II Sequencer and MiSeq system. Reads were de novo assembled using Celera Assembler v.8.0. Genome annotation was performed using the NCBI Prokaryotic Genome Annotation Pipeline (PGAP), and the genome sequence was analysed by bioinformatics methods.

Results: The 2963729-bp genome with a G+C content of 41.30% displayed 11 antimicrobial resistance genes, including bla_NDM-1 and bla_OXA-58. Meanwhile, 2 plasmids and 19 genomic islands were predicted within the genome.

Conclusion: The whole-genome sequence reported here can be compared with other genomes of NDM-1-producing Acinetobacter spp. These data could facilitate further understanding of the specific genomic features of carbapenem-resistant Acinetobacter spp. in China.