Draft genome sequence of a uropathogenic Escherichia coli isolate (ST38 O1:H15) from Pakistan, an emerging multidrug-resistant sequence type with a high virulence profile

Z Rafaque; J I Dasti; S C Andrews

doi:10.1016/j.nmni.2018.10.004

Draft genome sequence of a uropathogenic Escherichia coli isolate (ST38 O1:H15) from Pakistan, an emerging multidrug-resistant sequence type with a high virulence profile

New Microbes New Infect. 2018 Oct 19:27:1-2. doi: 10.1016/j.nmni.2018.10.004. eCollection 2019 Jan.

Authors

Z Rafaque^{1

2}, J I Dasti¹, S C Andrews²

Affiliations

¹ Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
² School of Biological Sciences, University of Reading, Reading, United Kingdom.

Abstract

Sequence type 38 is considered a uropathogenic Escherichia coli/enteroaggregative E. coli hybrid associated with multidrug resistance and urinary tract infections. The draft genome sequence of UEC59 from a woman in Pakistan revealed a 5 324 938 bp genome with 5386 coding sequences (CDS), 86 transfer RNA genes and multiple antibiotic resistance genes (bla _TEM-1, CMY-2, sul1, sul2, dfrA17, tetA, mphA) and mobile elements (int1, two transposons, 30 insertion sequence elements, one integrative conjugative element, four plasmids, five prophages), along with many virulence genes.

Keywords: Draft genome; MDR; Pakistan; ST38; UPEC; virulence.