Abstract
A gene coding for human stefin B was synthesized by the solid-phase phosphite method and cloned in the pUC8 cloning vector. The insert with the verified DNA sequence was subcloned into two expression vectors and expressed in E. coli as a fusion protein with beta-galactosidase and as a native protein. The CNBr cleaved fusion protein and the native recombinant stefin B were inhibitory to papain and reacted with antibodies against human stefin B.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cloning, Molecular*
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Cystatin B
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Cystatins*
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Escherichia coli / genetics*
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Genes*
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Genes, Synthetic*
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Humans
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Molecular Sequence Data
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Protease Inhibitors / genetics*
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Proteins / genetics*
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Proteins / isolation & purification
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Recombinant Proteins / isolation & purification
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Transcription, Genetic
Substances
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CSTB protein, human
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Cystatins
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Protease Inhibitors
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Proteins
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Recombinant Proteins
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Cystatin B