Self-assembled CpG oligodeoxynucleotides conjugated hollow gold nanospheres to enhance cancer-associated immunostimulation

Colloids Surf B Biointerfaces. 2019 Mar 1:175:248-255. doi: 10.1016/j.colsurfb.2018.12.001. Epub 2018 Dec 3.

Abstract

To realize efficiently cellular uptake and enhance the immunostimulation, thiolated cytosine-phosphate-guanine (CpG) oligodeoxynucleotides (ODNs) were self-assembled on hollow gold nanospheres (HGNs) to form CpG-HGNs. The cellular uptake of CpG-HGNs in immune cells was studied in RAW 264.7 cells. Due to the enhanced delivery efficiency, CpG-HGNs exhibited a higher immune stimulatory activity compared with CpG ODNs, resulting in a dramatically enhanced secretion of proinflammatory cytokines. In addition, CpG-HGNs showed low cytotoxicity in RAW 264.7 cells. CpG-HGNs could potentially realize synergistic photothermal therapy and immunotherapy in vivo.

Keywords: CpG oligodeoxynucleotide; Hollow gold nanosphere; Immunostimulation; Photothermal; Self-assembled.

MeSH terms

  • Animals
  • Cell Survival / drug effects
  • Cell Survival / radiation effects
  • Cytokines / metabolism
  • Gold / chemistry*
  • Humans
  • Inflammation Mediators / metabolism
  • Lasers
  • Mice
  • Microscopy, Electron, Transmission
  • Nanospheres / administration & dosage
  • Nanospheres / chemistry*
  • Nanospheres / ultrastructure
  • Neoplasms / metabolism
  • Neoplasms / therapy
  • Oligodeoxyribonucleotides / chemistry*
  • RAW 264.7 Cells
  • Temperature

Substances

  • Cytokines
  • Inflammation Mediators
  • Oligodeoxyribonucleotides
  • Gold