Mitomycin C-induced bidirectional transcription from the colicin E1 promoter region in plasmid ColE1

Biochim Biophys Acta. 1986 Oct 16;868(1):39-44. doi: 10.1016/0167-4781(86)90084-9.

Abstract

Treatment of a colicinogenic culture with mitomycin C induces convergent transcription from two adjacent promoters at the beginning of the colicin E1 gene. S1-mapping and primer extension assays indicate that the mitomycin C-inducible transcripts correspond to colicin E1 mRNA (cea mRNA) and to a transcript, designated RNA-C, that may code for an entry exclusion function. Nucleotide sequences that strongly resemble a consensus sequence for LexA protein binding sites span the transcription start points for cea mRNA and RNA-C. These putative operator sequences overlap by one base pair and bind LexA protein (Ebina, Y., Takahara, Y., Kishi, F., Nakazawa, A. and Brent, R. (1983) J. Biol. Chem. 258, 13258-13261). The data suggest that mitomycin C-induced bidirectional transcription from the cea mRNA and RNA-C promoters is controlled by the SOS regulatory system of Escherichia coli.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacteriocin Plasmids / drug effects*
  • Escherichia coli / drug effects
  • Escherichia coli / genetics
  • Mitomycin
  • Mitomycins / pharmacology*
  • Plasmids / drug effects*
  • Promoter Regions, Genetic / drug effects*
  • RNA, Messenger / metabolism
  • SOS Response, Genetics / drug effects
  • Transcription, Genetic / drug effects*

Substances

  • Mitomycins
  • RNA, Messenger
  • Mitomycin