Hemopoietic Cell Kinase amplification with Protein Tyrosine Phosphatase Receptor T depletion leads to polycythemia, aberrant marrow erythoid maturation, and splenomegaly

Sci Rep. 2019 May 7;9(1):7050. doi: 10.1038/s41598-019-43373-6.

Abstract

Deletion of long arm of chromosome 20 [del(20q)] is the second most frequent recurrent chromosomal abnormality in hematological malignancies. It is detected in 10% of myeloproliferative neoplasms, 4-5% of myelodysplastic syndromes, and 1-2% of acute myeloid leukaemia. Recurrent, non-random occurrence of del(20q) indicates that it is a pathogenic driver in myeloid malignancies. Genetic mapping of patient samples has identified two regions of interest on 20q - the "Common Deleted Region" (CDR) and "Common Retained Region" (CRR), which was often amplified. We proposed that the CDR contained tumor suppressor gene(s) (TSG) and the CRR harbored oncogene(s); loss of a TSG together with over-expression of an oncogene favored development of myeloid malignancies. Protein Tyrosine Phosphatase Receptor T (PTPRT) and Hemopoietic cell kinase (HCK) were identified to be the likely candidate TSG and oncogene respectively. Retroviral transduction of HCK into PTPRT-null murine LKS+ stem and progenitor cells resulted in hyperproliferation in colony forming assays and hyperphosphorylation of intracellular STAT3. Furthermore, over half of the murine recipients of these transduced cells developed erythroid hyperplasia, polycythemia and splenomegaly at 12 months, although no leukemic phenotype was observed. The findings suggested that HCK amplification coupled with PTPRT loss in del(20q) leads to development of a myeloproliferative phenotype.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Marrow / pathology
  • Erythropoiesis / physiology*
  • Gene Expression Regulation
  • Hematopoietic Stem Cells / pathology
  • Hematopoietic Stem Cells / physiology
  • Mice, Inbred C57BL
  • Mice, Mutant Strains
  • Oncogenes
  • Polycythemia / genetics*
  • Proto-Oncogene Proteins c-hck / genetics*
  • Proto-Oncogene Proteins c-hck / metabolism
  • Receptor-Like Protein Tyrosine Phosphatases, Class 2 / genetics*
  • Receptor-Like Protein Tyrosine Phosphatases, Class 2 / metabolism
  • STAT3 Transcription Factor / metabolism
  • Splenomegaly / etiology*
  • Splenomegaly / pathology

Substances

  • STAT3 Transcription Factor
  • Stat3 protein, mouse
  • Hck protein, mouse
  • Proto-Oncogene Proteins c-hck
  • Ptprt protein, mouse
  • Receptor-Like Protein Tyrosine Phosphatases, Class 2