CircSAMD4A accelerates cell proliferation of osteosarcoma by sponging miR-1244 and regulating MDM2 mRNA expression

Biochem Biophys Res Commun. 2019 Aug 13;516(1):102-111. doi: 10.1016/j.bbrc.2019.05.182. Epub 2019 Jun 11.

Abstract

Here, we firstly examined the proliferation and invasion capacities of different osteosarcoma (OS) cell lines, and analyzed the profiling of the differentially expressed circular RNAs(circRNAs). Then, we identified a novel circRNA(circ_101356/circ_0004846, we named circSAMD4 in the present study) and found it was highly expressed in the OS tissues compared to adjacent non-cancerous tissues. Furthermore, we indicated that circSAMD4A promoted OS proliferation in vivo and in vitro. In addition, we proved that circSAMD4A enhances osteosarcoma cells stemness features. In mechanism, circSAMD4A could sponge miR-1244 in OS cells. Moreover, we verified that MDM2 was a target of miR-1244. In conclusion, circSAMD4A/miR-1244/MDM2 regulatory loop might be a promising therapeutic target for OS treatment.

Keywords: MDM2; Osteosarcoma; Proliferation; circSAMD4A; miR-1244.

MeSH terms

  • Animals
  • Bone Neoplasms / genetics*
  • Cell Line, Tumor
  • Cell Proliferation
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Mice, Inbred BALB C
  • Mice, Nude
  • MicroRNAs / genetics*
  • Neoplasm Invasiveness / genetics
  • Osteosarcoma / genetics*
  • Proto-Oncogene Proteins c-mdm2 / genetics*
  • RNA, Circular / genetics*
  • RNA, Messenger / genetics

Substances

  • MIRN1244 microRNA, human
  • MicroRNAs
  • RNA, Circular
  • RNA, Messenger
  • MDM2 protein, human
  • Proto-Oncogene Proteins c-mdm2