Low level of exosomal long non-coding RNA HOTTIP is a prognostic biomarker in colorectal cancer

RNA Biol. 2019 Oct;16(10):1339-1345. doi: 10.1080/15476286.2019.1637697. Epub 2019 Jul 7.

Abstract

Molecular risk stratification of colorectal cancer can improve patient outcome. A panel of lncRNAs (H19, HOTTIP, HULC and MALAT1) derived from serum exosomes of patients with non-metastatic CRC and healthy donors was analyzed. Exosomes from healthy donors carried significantly more H19, HULC and HOTTIP transcripts in comparison to CRC patients. Correlation analysis between lncRNAs and clinical data revealed a statistical significance between low levels of exosomal HOTTIP and poor overall survival. This was confirmed by multivariate analysis that HOTTIP is an independent prognostic marker for overall survival (HR: 4.5, CI: 1.69-11.98, p = 0.0027). Here, HOTTIP poses to be a valid biomarker for patients with a CRC to predict post-surgical survival time.

Keywords: Exosomes; colorectal cancer; diagnosis; liquid biopsy; long non-coding RNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers, Tumor*
  • Case-Control Studies
  • Colorectal Neoplasms / diagnosis*
  • Colorectal Neoplasms / genetics*
  • Colorectal Neoplasms / metabolism
  • Colorectal Neoplasms / mortality
  • Exosomes / metabolism
  • Exosomes / ultrastructure
  • Gene Expression Profiling
  • Humans
  • Liquid Biopsy
  • Neoplasm Staging
  • Prognosis
  • Proportional Hazards Models
  • RNA, Long Noncoding / genetics*
  • ROC Curve

Substances

  • Biomarkers, Tumor
  • H19 long non-coding RNA
  • RNA, Long Noncoding
  • long noncoding RNA HOTTIP, human

Grants and funding

This work was supported by the Deutsche Forschungsgemeinschaft [KA 3511/3–1]; Nemzeti Kutatási Fejlesztési és Innovációs Hivatal (NKFIH) [NVKP_16–1–2016–0037]; Nemzeti Kutatási Fejlesztési és Innovációs Hivatal (NKFIH) [KH-129581]; Roland Ernst Foundation [5/15].