Normal B cell development and Pax5 expression in Thy28/ThyN1-deficient mice

Fusako Kitaura; Miyuki Yuno; Toshitsugu Fujita; Shigeharu Wakana; Jun Ueda; Kazuo Yamagata; Hodaka Fujii

doi:10.1371/journal.pone.0220199

Normal B cell development and Pax5 expression in Thy28/ThyN1-deficient mice

PLoS One. 2019 Jul 22;14(7):e0220199. doi: 10.1371/journal.pone.0220199. eCollection 2019.

Authors

Fusako Kitaura¹, Miyuki Yuno¹, Toshitsugu Fujita^{1

2}, Shigeharu Wakana^{3

4}, Jun Ueda^{5

6}, Kazuo Yamagata^{5

7}, Hodaka Fujii^{1

2}

Affiliations

¹ Chromatin Biochemistry Research Group, Combined Program on Microbiology and Immunology, Research Institute for Microbial Diseases, Osaka University, Yamadaoka, Suita, Osaka, Japan.
² Department of Biochemistry and Genome Biology, Hirosaki University Graduate School of Medicine, Zaifu-cho, Hirosaki, Aomori, Japan.
³ Technology and Development Team for Mouse Phenotype Analysis, RIKEN BioResource Research Center, Koyadai, Tsukuba, Ibaraki, Japan.
⁴ Department of Gerontology, Institute of Biomedical Research and Innovation, Minatojima Minamiachi, Chuo-ku, Kobe, Japan.
⁵ Center for Genetic Analysis of Biological Responses, Research Institute for Microbial Diseases, Osaka University, Yamadaoka, Suita, Japan.
⁶ Center for Advanced Research and Education (CARE), Asahikawa Medical University, Asahikawa, Japan.
⁷ Faculty of Biology-Oriented Science and Technology, Kindai University, Nishimitani, Kinokawa City, Wakayama, Japan.

Abstract

Thy28, also known as ThyN1, is a highly conserved nuclear protein. We previously showed that in a chicken mature B cell line, Thy28 binds to the promoter of the gene encoding Pax5, a transcription factor essential for B cell development, and positively regulates its expression. Here, we generated a Thy28-deficient mouse line to analyze its potential role in B cell development in mice. Thy28-deficient mice showed normal development of B cells, and the expression of Pax5 was comparable between wild-type and Thy28-deficient primary B cells. Thus, species-specific mechanisms regulate Pax5 expression and B cell development.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
B-Lymphocytes / cytology
B-Lymphocytes / metabolism*
Female
Male
Mice
Mice, Inbred C57BL
Nuclear Proteins / deficiency*
Nuclear Proteins / genetics
PAX5 Transcription Factor / genetics*
PAX5 Transcription Factor / metabolism

Substances

Nuclear Proteins
PAX5 Transcription Factor
Pax5 protein, mouse
Thy28 protein, mouse

Grants and funding

This work was supported by the Takeda Science Foundation (T.F.), Grant-in-Aid for Scientific Research (C) (#15K06895) (T.F.), and Grant-in-Aid for Scientific Research (B) (#15H04329) (T.F., H.F.), ‘Transcription Cycle’ (#15H01354) (H.F.) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.