FZD10 Carried by Exosomes Sustains Cancer Cell Proliferation

Cells. 2019 Jul 25;8(8):777. doi: 10.3390/cells8080777.

Abstract

Extracellular vesicles (EVs) are involved in intercellular communication during carcinogenesis, and cancer cells are able to secrete EVs, in particular exosomes containing molecules, that can be transferred to recipient cells to induce pathological processes and significant modifications, as metastasis, increase of proliferation, and carcinogenesis evolution. FZD proteins, a family of receptors comprised in the Wnt signaling pathway, play an important role in carcinogenesis of the gastroenteric tract. Here, a still unknown role of Frizzled 10 (FZD10) protein was identified. In particular, the presence of FZD10 and FZD10-mRNA in exosomes extracted from culture medium of the untreated colorectal, gastric, hepatic, and cholangio cancer cell lines, was detected. A substantial reduction in the FZD10 and FZD10-mRNA level was achieved in FZD10-mRNA silenced cells and in their corresponding exosomes. Concomitantly, a significant decrease in viability of the silenced cells compared to their respective controls was observed. Notably, the incubation of silenced cells with the exosomes extracted from culture medium of the same untreated cells promoted the restoration of the cell viability and, also, of the FZD10 and FZD10-mRNA level, thus indicating that the FZD10 and FZD10-mRNA delivering exosomes may be potential messengers of cancer reactivation and play an active role in long-distance metastatization.

Keywords: FZD10 protein; FZD10-mRNA; FZD10-mRNA silenced cells; cell proliferation; cholangiocarcinoma cells; colorectal cancer cells; exosomes; gastric cancer cells; hepatocarcinoma cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Cell Proliferation
  • Cell Survival
  • Cells, Cultured
  • Exosomes / metabolism*
  • Extracellular Vesicles / metabolism
  • Fluorescent Antibody Technique
  • Frizzled Receptors / metabolism*
  • Humans
  • Neoplasms / metabolism*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism

Substances

  • FZD10 protein, human
  • Frizzled Receptors
  • RNA, Messenger