Chemical composition and anti-inflammatory activity of the essential oil from the leaves of Limnocitrus littoralis (Miq.) Swingle from Vietnam

Nat Prod Res. 2021 May;35(9):1550-1554. doi: 10.1080/14786419.2019.1652286. Epub 2019 Aug 20.

Abstract

The oil from the leaves of Limnocitrus littoralis (Miq.) Swingle was obtained by hydrodistillation and investigated by gas chromatography combined with mass spectrometry (GC/MS), the anti-inflammatory effect of the oil was examined on a LPS-induced RAW264.7 cells. An aggregate of forty components were identified, representing 93.0% of the oil. This oil was subjugated by monoterpene hydrocarbons (27.7%), sesquiterpene hydrocarbons (32.3%) and oxygenated sesquiterpenes (4.6%). The significant constituents of L. littoralis essential oil were determined as follows; myrcene (24.9%), γ-muurolene (11.0%), and oleic acid (10.3%). The essential oil of L. littoralis showed activity against the nitric oxide (NO) generation with the IC50 value to 12.50 ± 1.19 µg/L. The anti-inflammatory effect of essential oil from the leaves of L. littoralis is reported for the first time.

Keywords: Limnocitrus littoralis (Miq.) Swingle; anti-inflammatory activity; essential oil.

MeSH terms

  • Acyclic Monoterpenes / pharmacology
  • Alkenes / pharmacology
  • Animals
  • Anti-Inflammatory Agents / pharmacology*
  • Gas Chromatography-Mass Spectrometry
  • Mice
  • Monoterpenes / analysis
  • Nitric Oxide / biosynthesis
  • Oils, Volatile / chemistry*
  • Oils, Volatile / pharmacology*
  • Plant Leaves / chemistry*
  • RAW 264.7 Cells
  • Rutaceae / chemistry*
  • Sesquiterpenes / chemistry
  • Sesquiterpenes / pharmacology
  • Vietnam

Substances

  • Acyclic Monoterpenes
  • Alkenes
  • Anti-Inflammatory Agents
  • Monoterpenes
  • Oils, Volatile
  • Sesquiterpenes
  • Nitric Oxide
  • myrcene