The influence of gene polymorphisms on postprandial triglyceride response after oral fat tolerance test meal in patients with diabetes mellitus

Paraskevi Gavra; Vana Kolovou; Athanasia K Papazafiropoulou; Andreas Melidonis; Stella Iraklianou; Sophie Mavrogeni; Genovefa Kolovou

doi:10.1111/ijcp.13432

The influence of gene polymorphisms on postprandial triglyceride response after oral fat tolerance test meal in patients with diabetes mellitus

Int J Clin Pract. 2019 Oct 4:e13432. doi: 10.1111/ijcp.13432. Online ahead of print.

Authors

Paraskevi Gavra¹, Vana Kolovou^{2

3}, Athanasia K Papazafiropoulou¹, Andreas Melidonis¹, Stella Iraklianou¹, Sophie Mavrogeni³, Genovefa Kolovou³

Affiliations

¹ Tzanio General Hospital, Piraeus, Greece.
² Molecular Immunology Laboratory, Onassis Cardiac Surgery Center, Athens, Greece.
³ Cardiology Department, Onassis Cardiac Surgery Center, Athens, Greece.

PMID: 31585025
DOI: 10.1111/ijcp.13432

Abstract

Aims: We evaluated the influence of CETP (rs5882 and rs708272), APOE (rs7412, rs429358) and LPL (rs328) gene polymorphisms on triglyceride (TG) response to oral fat tolerance test (OFTT) meal in patients with well-controlled type 2 diabetes mellitus (T2DM).

Methods: Fifty-one men underwent OFTT and according to postprandial TG response patients were divided into two subgroups (positive [TG ≥ 220 mg/dL, 31 patients] and negative [TG < 220 mg/dL, 20 patients]). All patients were genotyped, and study variants were detected using polymerase chain reaction (PCR) and restricted fragment length polymorphism (RFLP) analysis.

Results: Patients with genotype SS of LPL gene compared with genotype SX had more frequently positive response to OFTT (P = .04) and lower high-density lipoprotein cholesterol (HDL-C) concentration (P = .03). Patients with positive response to OFTT and genotype SS of LPL gene compared with genotype SX had lower AUC (area under the curve)-TG, 1744 (368) vs 1887 (807) mg/dL/h, respectively, P = .04. CETP and APOE gene polymorphisms had no influence on postprandial TG response to OFTT.

Conclusions: In patients with well-controlled T2DM, LPL but not CETP and APOE gene polymorphisms influenced TG postprandial response. Particularly, S447 allele carriers of LPL gene presented more frequently positive postprandial TG response to OFTT compared with 447X allele carriers. No differences were found between allele carriers of patients with negative response to OFTT in any other studied gene polymorphism.