Multigene phylogeny and taxonomy of Dendryphion hydei and Torula hydei spp. nov. from herbaceous litter in northern Thailand

Junfu Li; Rajesh Jeewon; Peter E Mortimer; Mingkwan Doilom; Rungtiwa Phookamsak; Itthayakorn Promputtha

doi:10.1371/journal.pone.0228067

Multigene phylogeny and taxonomy of Dendryphion hydei and Torula hydei spp. nov. from herbaceous litter in northern Thailand

PLoS One. 2020 Feb 5;15(2):e0228067. doi: 10.1371/journal.pone.0228067. eCollection 2020.

Authors

Junfu Li^{1

2

3

4}, Rajesh Jeewon⁵, Peter E Mortimer³, Mingkwan Doilom^{3

6}, Rungtiwa Phookamsak^{3

4

6}, Itthayakorn Promputtha^{1

2}

Affiliations

¹ Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand.
² Center of Excellence in Bioresources for Agriculture, Industry and Medicine, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand.
³ Key Laboratory of Biodiversity and Biogeography, Chinese Academy of Sciences, Kunming Institute of Botany, Kunming, P.R. China.
⁴ Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, Thailand.
⁵ Department of Health Sciences, Faculty of Science, University of Mauritius, Reduit, Mauritius.
⁶ World Agroforestry Centre, East and Central Asia, Kunming, P.R. China.

Abstract

During our studies on asexual fungi colonizing herbaceous litter in northern Thailand, we discovered two new fungal species, viz. Dendryphion hydei and Torula hydei spp. nov. The latter are examined, and their morphological characters are described as well as their DNA sequences from ribosomal and protein coding genes are analysed to infer their phylogenetic relationships with extant fungi. Torula hydei is different from other similar Torula species in having tiny and catenate conidia. Dendryphion hydei can be distinguished from other similar Dendryphion species in having large conidiophores and subhyaline to pale olivaceous brown, 2-4(-5)-septate conidia. Multigene phylogenetic analyses of a combined LSU, SSU, TEF1-α, RPB2 and ITS DNA sequence dataset generated from maximum likelihood and Bayesian inference analyses indicate that T. hydei forms a distinct lineage and basal to T. fici. Dendryphion hydei forms a distinct lineage and basal to D. europaeum, D. comosum, D. aquaticum and D. fluminicola within Torulaceae (Pleosporales, Dothideomycetes).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ascomycota / classification
Ascomycota / genetics*
Ascomycota / physiology
Bayes Theorem
Cryptococcus / classification
Cryptococcus / genetics*
Cryptococcus / physiology
DNA, Fungal / chemistry
DNA, Fungal / metabolism
Phylogeny
Sequence Analysis, DNA
Thailand

Substances

DNA, Fungal

Grants and funding

The authors are grateful the Mushroom Research Foundation, Chiang Mai, Thailand and the Key Research Program of Frontier Sciences of the Chinese Academy of Sciences (grant no. QYZDY-SSW-SMC014) for supporting this research. Rungtiwa Phookamsak thanks CAS President’s International Fellowship Initiative (PIFI) for young staff (grant no. Y9215811Q1) and National Science Foundation of China (NSFC) project code 31850410489 (grant no. Y81I982211) for financial support. Peter E Mortimer would like to thank the National Science Foundation of China and the South East Asian Biodiversity resources Institute, Chinese Academy of Sciences, for financial support under the following grants: 41761144055, 41771063, Y4ZK111B01. Mingkwan Doilom thanks the 5th batch of Postdoctoral Orientation Training Personnel in Yunnan Province and the 64th batch of China Postdoctoral Science Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.