Graphdiyne nanoradioprotector with efficient free radical scavenging ability for mitigating radiation-induced gastrointestinal tract damage

Jiani Xie; Chengyan Wang; Ning Wang; Shuang Zhu; Linqiang Mei; Xiao Zhang; Yuan Yong; Lele Li; Chunying Chen; Changshui Huang; Zhanjun Gu; Yuliang Li; Yuliang Zhao

doi:10.1016/j.biomaterials.2020.119940

Graphdiyne nanoradioprotector with efficient free radical scavenging ability for mitigating radiation-induced gastrointestinal tract damage

Biomaterials. 2020 Jun:244:119940. doi: 10.1016/j.biomaterials.2020.119940. Epub 2020 Mar 6.

Authors

Jiani Xie¹, Chengyan Wang², Ning Wang³, Shuang Zhu⁴, Linqiang Mei², Xiao Zhang⁴, Yuan Yong⁵, Lele Li⁶, Chunying Chen⁶, Changshui Huang⁷, Zhanjun Gu⁸, Yuliang Li⁹, Yuliang Zhao¹⁰

Affiliations

¹ College of Pharmacy and Biological Engineering, Chengdu University, Chengdu, 610106, China; CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, 100049, China.
² CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, 100049, China; Center of Materials Science and Optoelectronics Engineering, College of Materials Science and Optoelectronic Technology, University of Chinese Academy of Sciences, Beijing, 100049, China.
³ Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China.
⁴ CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, 100049, China.
⁵ College of Chemistry and Environment Protection Engineering, Southwest Minzu University, Chengdu, 610041, China.
⁶ CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China, Chinese Academy of Sciences, Beijing, 100190, China.
⁷ Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China. Electronic address: huangcs@qibebt.ac.cn.
⁸ CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, 100049, China; Center of Materials Science and Optoelectronics Engineering, College of Materials Science and Optoelectronic Technology, University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic address: zjgu@ihep.ac.cn.
⁹ Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
¹⁰ Center of Materials Science and Optoelectronics Engineering, College of Materials Science and Optoelectronic Technology, University of Chinese Academy of Sciences, Beijing, 100049, China; CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China, Chinese Academy of Sciences, Beijing, 100190, China. Electronic address: zhaoyl@nanoctr.cn.

PMID: 32200103
DOI: 10.1016/j.biomaterials.2020.119940

Abstract

X-ray irradiation-induced toxicity to gastrointestinal tract become a significant clinical problem when using radiotherapy for treating abdominal tumors neighbored to gastrointestinal tissue, which not only often prevents these tumors from receiving a definitive therapeutic dose but also causes a series of gastrointestinal diseases, such as anorexia, abdominal pain, diarrhea and hematochezia. And thus it seriously reduces the therapeutic outcome and life quality of patients. Therefore, the development of gastrointestinal radioprotectors is essential. However, the commercial gastrointestinal radioprotectors in clinical are still rare. In view of this, we prepared bovine serum albumin (BSA) modified graphdiyne (GDY) nanoparticles (GDY-BSA NPs) and for the first time studied its gastrointestinal radioprotection ability. The unique advantages of GDY nanomaterial, including high free radical scavenging ability, good chemical stability in gastric acid condition, relatively longer residence time in gastrointestinal tract and good biosafety under oral administration, provide the favorable prerequisites for it to be used as the gastrointestinal radioprotector. In vitro experimental results indicated that the GDY-BSA NPs powerfully reduced DNA damage and improved viability of the irradiated gastrointestinal cells. In vivo results showed that the GDY-BSA NPs significantly decrease radiation-induced diarrhea, weight loss, and gastrointestinal tissue pathological damage of mice. Furthermore, we also deeply studied the gastrointestinal radioprotective mechanism of GDY-BSA NPs, which indicated that the GDY-BSA NPs effectively inhibited reactive oxygen species (ROS)-induced apoptosis signal pathway, and thus reduced gastrointestinal cell apoptosis. Our work for the first time employed BSA-GDY NPs to mitigating radiation-induced gastrointestinal tract damage, which not only promotes the exploration of new gastrointestinal tract radioprotectors, but also is the good guidance for the treatment of gastrointestinal diseases by nano-drug.

Keywords: Free radical scavenging; Gastrointestinal tract; Graphdiyne; Nanoradioprotector; Oral activity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Free Radicals
Gastrointestinal Tract
Graphite*
Humans
Mice
Serum Albumin, Bovine

Substances

Free Radicals
graphdiyne
Serum Albumin, Bovine
Graphite