Generation of a hiPSC line ZZUNEUi017-A from a patient with dilated cardiomyopathy caused by mutation in TTN

Shujian Huang; Wanrong Fu; Guangli Guo; Xiaoxu Tiana; Xiaoyan Zhao; Jianzeng Dong; Xiaowei Li; Haibo Yang

doi:10.1016/j.scr.2021.102248

Generation of a hiPSC line ZZUNEUi017-A from a patient with dilated cardiomyopathy caused by mutation in TTN

Stem Cell Res. 2021 Apr:52:102248. doi: 10.1016/j.scr.2021.102248. Epub 2021 Feb 15.

Authors

Shujian Huang¹, Wanrong Fu¹, Guangli Guo¹, Xiaoxu Tiana¹, Xiaoyan Zhao¹, Jianzeng Dong², Xiaowei Li³, Haibo Yang⁴

Affiliations

¹ Centre for Cardiovascular Diseases, Henan Key Laboratory of Hereditary Cardiovascular Diseases, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou 450052, People's Republic of China.
² Centre for Cardiovascular Diseases, Henan Key Laboratory of Hereditary Cardiovascular Diseases, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou 450052, People's Republic of China; Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, National Clinical Research Centre for Cardiovascular Diseases, No.2 Beijing Anzhen Road, Chaoyang District, Beijing 100029, China.
³ Centre for Cardiovascular Diseases, Henan Key Laboratory of Hereditary Cardiovascular Diseases, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou 450052, People's Republic of China. Electronic address: fcclixw@zzu.edu.cn.
⁴ Centre for Cardiovascular Diseases, Henan Key Laboratory of Hereditary Cardiovascular Diseases, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou 450052, People's Republic of China. Electronic address: yanghaibo1975@126.com.

PMID: 33610015
DOI: 10.1016/j.scr.2021.102248

Abstract

Dilated cardiomyopathy (DCM) is the commonest type of cardiomyopathy. In this study, peripheral blood mononuclear cells (PBMCs) were isolated from a DCM patient with the p. Glu12513fs(c.37537delG) mutation in TTN and were reprogrammed to human induced pluripotent stem cells (iPSCs). The ZZUNEUi017-A iPSC line expressed pluripotency markers, exhibiting a normal male karyotype (46, XY) and demonstrating differentiation potential into three germ layers in vitro.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cardiomyopathy, Dilated* / genetics
Cell Differentiation
Connectin
Humans
Induced Pluripotent Stem Cells*
Leukocytes, Mononuclear
Male
Mutation

Substances

Connectin
TTN protein, human