Objectives: To investigate the expression of cyclophilin A (CyPA) in oral squamous cell carcinoma (OSCC) and explore the effect of downregulating the expression of CyPA gene on the proliferation and invasion of SCC-25 cells.
Methods: A total of 77 cases of patients with OSCC were selected. The expression levels of CyPA proteins in OSCC and adjacent normal tissues were evaluated. SCC-25 cells were cultured and divided into the CyPA interference sequence group, negative control group, and blank group. The expression levels of CyPA mRNA and protein in cells were detected by using real-time fluorescent quantitative polymerase chain reaction and Western blot, respectively. Cell proliferation was detected by using methyl thiazolyl tetrazolium and plate colony formation assays. Cell invasion was detected by using Transwell assay.
Results: The positive expression rate of CyPA protein in OSCC tissues was 76.62%, which was higher than that in adjacent tissues (P<0.05). The positive expression rate of CyPA protein in TNM stage T3+T4, clinical stage Ⅲ+Ⅳ, moderately or poorly differentiated lymph node metastasis was increased (P<0.05). Compared with the negative control and blank groups, the CyPA interference sequence group had decreased relative expression levels of CyPA mRNA and protein (P<0.05); optical density va-lues of cells at 24, 48, 72, and 96 h (P<0.05); and number of cell colonies and invasive cells (P<0.05).
Conclusions: The CyPA protein is highly expressed in OSCC tissues, and the downregulation of CyPA gene expression in SCC-25 cells can reduce cell proliferation and inhibit cell invasion.
目的: 探讨亲环蛋白A（CyPA）在口腔鳞状细胞癌（OSCC）组织中的表达，以及下调SCC-25细胞中CyPA基因对细胞增殖和侵袭的影响。方法: 选取OSCC患者77例，检测OSCC及癌旁组织中CyPA蛋白表达，培养SCC-25细胞并分为CyPA干扰序列组、阴性对照组和空白组，分别采用实时荧光定量聚合酶链反应技术和Western blot技术检测细胞中CyPA mRNA和蛋白表达，细胞增殖能力检测采用甲基噻唑基四唑（MTT）法和平板集落形成实验，细胞侵袭能力检测采用Transwell法。结果: OSCC组织中CyPA蛋白阳性表达率为76.62%，高于癌旁组织（P<0.05）；CyPA蛋白在TNM分期T3+T4期、临床分期Ⅲ+Ⅳ期、中低分化和发生淋巴结转移组织中阳性表达率升高（P<0.05）；与阴性对照组和空白组比较，CyPA干扰序列组细胞中CyPA mRNA和蛋白相对表达量降低（P<0.05），24、48、72和96 h细胞光密度值降低（P<0.05），细胞集落数和侵袭细胞数均降低（P<0.05）。结论: CyPA蛋白在OSCC组织中呈高表达，下调SCC-25细胞中CyPA基因表达可抑制细胞增殖和侵袭能力。.
Keywords: cell proliferation; cyclophilin A; invasiveness; oral squamous cell carcinoma.