LncRNA MAFG-AS1 affects the tumorigenesis of breast cancer cells via the miR-574-5p/SOD2 axis

Biochem Biophys Res Commun. 2021 Jun 30:560:119-125. doi: 10.1016/j.bbrc.2021.04.133. Epub 2021 May 11.

Abstract

Amounting evidence suggested that long non coding RNAs (lncRNAs) played vital roles in the progression of various cancers. The aim of this study is to examine the biological roles and underlying mechanisms of lncRNA MAFG-AS1 in the tumorigenesis of breast cancer (BC) cells. Here we showed that downregulation of MAFG-AS1 inhibited the viability, migration, and invasion of BC cells. Mechanism investigation showed that inhibition of MAFG-AS1 induced apoptosis via the intrinsic apoptotic pathway and overexpression of Bcl-2 could inhibited it. Further, MAFG-AS1 acts as a sponge of miR-574-5p which directly binds to SOD2 mRNA. Re-expression of SOD2 using a 3'-UTR mutant SOD2 reversed the effects of silencing of MAFG-AS1 on BC cells. Finally, downregulation of MAFG-AS1 inhibited the growth of tumour in vivo. Together, MAFG-AS1 acts as an oncogene via regulation of miR-574-5p/SOD2 axis in BC cells.

Keywords: Breast cancer; MAFG-AS1; SOD2; miR-574-5p.

MeSH terms

  • Animals
  • Apoptosis
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / metabolism
  • Breast Neoplasms / pathology
  • Carcinogenesis
  • Caspases / metabolism
  • Cell Line, Tumor
  • Cell Movement
  • Cell Survival
  • Female
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Mice
  • Mice, SCID
  • MicroRNAs / metabolism*
  • Neoplasm Invasiveness
  • RNA, Long Noncoding / metabolism*
  • Superoxide Dismutase / genetics*
  • Superoxide Dismutase / metabolism

Substances

  • MIRN574 microRNA, human
  • MicroRNAs
  • RNA, Long Noncoding
  • Superoxide Dismutase
  • superoxide dismutase 2
  • Caspases