Hydrophobin HGFI assisted immunobiologic sensor based on a cascaded taper integrated ultra-long-period fiber grating

Binbin Song; Chang Jin; Bo Wang; Jixuan Wu; Bo Liu; Wei Lin; Wei Huang; Shaoxiang Duan; Mingqiang Qiao

doi:10.1364/BOE.425014

Hydrophobin HGFI assisted immunobiologic sensor based on a cascaded taper integrated ultra-long-period fiber grating

Biomed Opt Express. 2021 Apr 19;12(5):2790-2799. doi: 10.1364/BOE.425014. eCollection 2021 May 1.

Authors

Binbin Song¹, Chang Jin¹, Bo Wang², Jixuan Wu^{3

4}, Bo Liu⁵, Wei Lin⁵, Wei Huang¹, Shaoxiang Duan⁵, Mingqiang Qiao^{2

6}

Affiliations

¹ The Key Laboratory of Computer Vision and System of Ministry of Education, Tianjin Key Laboratory of Intelligence Computing and Novel Software Technology, Tianjin University of Technology, Tianjin, 300071, China.
² Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.
³ Tianjin Key Laboratory of Optoelectronic Detection Technology and Systems, Tianjin Key Laboratory of Engneering Technologies for Cell Phamaceutical, College of Electronic and Information Engineering, Tiangong University, Tianjin 300387, China.
⁴ wujixuan@tiangong.edu.cn.
⁵ Key Laboratory of Optoelectronic Sensor and Sensing Network Technology, College of Electronic Information and Optical Engineering, Nankai University, Tianjin 300350, China.
⁶ qiaomq@nankai.edu.cn.

Abstract

A new type of cascaded taper integrated ultra-long-period fiber grating (ULPFG) based immunobiologic sensor has been developed that benefits from the self-assembled monolayer of class I hydrophobin HGFI. Due to the cascaded arc, discharge tapers constitute an ultra-long-period and circular symmetrical refractive index modulation along fiber axial direction, and by local integration in one period, the mode coupling would generate to the higher harmonic of LP₀₂, LP₀₃ and LP₀₄ modes in the wavelength range from 1300 nm to 1620 nm. The hydrophobic characteristic of the ULPFG surface is modified employing the HGFI, and the antibody molecule probes could be absorbed strongly on the HGFI nano-film, furthermore, the performances of immunobiologic sensing are investigated employing multiple control groups of matched and mismatched antigen molecule targets. The results show that it possesses higher sensing sensitivity of 4.5 nm/(µg/ml), faster response time about of 35 min, lower stability error of 8.8%, and excellent immuno-specificity. Moreover, this proposed ULPFG sensor has the advantages of low cost, simple fabrication and label-free, which is a powerful tool in the trace biomedical detection field.