Amplification and analysis of DNA sequences in single human sperm and diploid cells

H H Li; U B Gyllensten; X F Cui; R K Saiki; H A Erlich; N Arnheim

doi:10.1038/335414a0

Amplification and analysis of DNA sequences in single human sperm and diploid cells

Nature. 1988 Sep 29;335(6189):414-7. doi: 10.1038/335414a0.

Authors

H H Li¹, U B Gyllensten, X F Cui, R K Saiki, H A Erlich, N Arnheim

Affiliation

¹ Department of Biological Sciences, University of Southern California, Los Angeles 90089-0371.

PMID: 3419517
DOI: 10.1038/335414a0

Abstract

The use of the polymerase chain reaction for analysing DNA sequences in individual diploid cells and human sperm shows that two genetic loci can be co-amplified from a single sperm, which may allow the analysis of previously inaccessible genetic phenomena.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Base Sequence
Cell Line
Chromosome Mapping
Chromosomes
DNA*
DNA-Directed DNA Polymerase
Gene Amplification*
Globins / genetics
HLA-DQ Antigens / genetics
Humans
Male
Nucleic Acid Hybridization
Receptors, LDL / genetics
Recombination, Genetic
Spermatozoa / ultrastructure*

Substances

HLA-DQ Antigens
Receptors, LDL
Globins
DNA
DNA-Directed DNA Polymerase