Immobilization of Lipase from Candida antarctica B (CALB) by Sol-Gel Technique Using Rice Husk Ash as Silic Source and Ionic Liquid as Additive

This work presents the immobilization in situ of commercial lipase from Candida antarctica B (CALB) by the sol-gel technique (xerogel) using silica from rice husk ash (RHA) as a source of silicon. It was used the Ionic Liquid (IL) 1-octyl-3-methylimidazolium bromide (C8MI.Br) as additive. The immobilized derivatives were characterized per SEM, XRD, and per method BET. The enzymatic activity of xerogels was evaluated with different tests, these being the reactional thermal analysis, immobilization yield, and operational and storage stability. The XDR showed that the obtained xerogels have halos in the region between 15 and 35° (2θ) what characterizes it as amorphous materials. The SEM analysis of xerogel shows irregular particles with dimensions less than 20 μm. The immobilized presented an esterification activity (EA) with 263.2 and 213.8 U/g, with and without IL, respectively, higher than the free enzyme (169.6 U/g). The immobilized, with and without IL, presented a significant improvement in the activity performance in relation to free enzyme for the three reactional temperatures (40, 60, and 80 °C) evaluated. The operational stability demonstrated that is possible to use xerogel without ionic liquid for 17 recycles and 21 recycles in IL presence. This methodology allows the preparation of new highly active and selective enzyme catalysts using the rice husk ash as a source of silicon, and the ionic liquid [C8MI]Br as additive. Furthermore, the new materials can provide greater viability in the processes, ensuring longer catalyst life.