Generation of Plvap-CreER and Car4-CreER for genetic targeting of distinct lung capillary populations

Zhenqian Zhang; Bin Zhou

doi:10.1016/j.jgg.2022.08.001

Generation of Plvap-CreER and Car4-CreER for genetic targeting of distinct lung capillary populations

J Genet Genomics. 2022 Dec;49(12):1093-1100. doi: 10.1016/j.jgg.2022.08.001. Epub 2022 Aug 23.

Authors

Zhenqian Zhang¹, Bin Zhou²

Affiliations

¹ State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences; University of Chinese Academy of Sciences, Shanghai 200031, China.
² State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences; University of Chinese Academy of Sciences, Shanghai 200031, China; Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, Zhejiang 310024, China; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China. Electronic address: zhoubin@sibs.ac.cn.

PMID: 36028133
DOI: 10.1016/j.jgg.2022.08.001

Abstract

It has been reported recently that there are two distinct subpopulations of capillary endothelial cells in the mammalian lungs: gCap (general capillary) and aCap (aerocyte). They are identified by two unique markers, respectively: plasmalemmal vesicle-associated protein (PLVAP) and carbonic anhydrase IV (CAR4). Here, we report two novel knock-in mouse lines Plvap-CreER and Car4-CreER, which genetically target gCap and aCap, respectively. Induced by tamoxifen, the Plvap-CreER and Car4-CreER alleles mediate specific and efficient Cre-loxP recombinations in PLVAP⁺ gCap and CAR4⁺ aCap, respectively, in the lungs. These two mouse lines are useful genetic tools to investigate cell fates and functions of PLVAP⁺ and CAR4⁺ cells in lung homeostasis, injury and repair.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alleles
Animals
Base Sequence
Cell Differentiation
Endothelial Cells* / metabolism
Lung* / metabolism
Mammals / genetics
Membrane Proteins / genetics
Mice

Substances

Plvap protein, mouse
Membrane Proteins