First Report of Diaporthe fusicola Causing Postharvest Fruit Rot of Peach (Prunus persica) in China

In May 2022, rot symptoms were observed on postharvest peach (Prunus persica [L.] Batsch) fruits in a market in Nanchang, Jiangxi province (28°44' N; 115°50' E), China. A total of 80 samples were collected from three different fruit stalls through the market survey. The incidence of this disease was 10 to 15%, and severity varies from approximately 30 to 50% of fruit surface coverage. The symptom of infected fruits was circular, pale brown to brown, rotten, necrotic lesions, covered with white hyphae and small spore masses. Eight symptomatic peach fruits were surface disinfected with 75% ethanol for 30 sec and incisions were made with a sterile scalpel. Small pieces from symptomatic tissues were placed on a potato dextrose agar (PDA) medium and incubated at 25℃ for 7 days. Six isolates were obtained in total. Colonies on PDA were initially white, aerial, fluffy at first, and darkened with age. Alpha conidia were fusoid, hyaline, aseptate, guttulate, tapering towards ends, and ranged in size from 9.8 to 5.1 µm × 3.2 to 2.1 µm (x ̅=7.1 ± 1.0 × 2.6 ± 0.3 µm, n=60). Beta conidia were not seen. For further confirmation, genomic DNA was extracted from three isolates (04-10, 04-11, and 04-12), the internal transcribed spacer (ITS) region, beta-tubulin (TUB), calmodulin (CAL), partial translation elongation factor 1-alpha (TEF1-α) and histone H3 (HIS) genes were amplified by using primers ITS1/ITS4, Bt2a/Bt2b, CAL228F/CAL737R, EF1-728F/EF1-986R, CYLH3F/H3-1b (Udayanga et al. 2015), respectively. Sequences were deposited in GenBank (Accession Nos. ON994257 to ON994259 for ITS, OP076824 to OP076826 for TUB, OP076827 to OP076829 for CAL, OP076821 to OP076823 for TEF1-α, OP076830 to OP076832 for HIS). BLAST results showed that ITS and TEF1-α have 99.8% pairwise identity to Diaporthe fusicola (MN816432, KF576256), and the TUB, CAL, and HIS sequences also have 100% pairwise identity to D. fusicola (KF576287, MT978147, MT978142). Phylogenetic analyses of concatenated sequences using Bayesian inference and the maximum likelihood confirmed the identity. To verify Koch's postulates, the pathogenicity of three isolates was tested on harvested healthy peach fruits. Five surface-sterilized fruits were wounded by a sterile scalpel and inoculated with 5-mm-diameter mycelial plugs from 10-day-old PDA plates. Another set of five fruits was inoculated with sterilized PDA plugs as controls. All fruits were incubated at 26℃ with 80% relative humidity. The experiment was repeated three times. After 5 days, the fruit inoculated with mycelial plugs showed pale brown lesions with whitish mycelium mass, similar to the previous rot symptoms, whereas the control fruit remained symptomless. The same pathogen was reisolated from the inoculated fruit with symptoms and identified as D. fusicola by molecular techniques, but never from the control. Diaporthe fusicola (Diaporthe amygdali complex) was first described on leaves of Lithocarpus glabra in China (Gao et al. 2015) and reported as an agent causing leaf blotch on Osmanthus fragrans (Si et al. 2020) and pear shoot canker (Guo et al. 2020). However, this is the first report of D. fusicola causing postharvest fruit rot on peach. The managers involved must consider the impact of this disease and develop an effective fruit storage strategy.