Characterization of a silent gene for human pancreatic elastase I: structure of the 5'-flanking region

J Biochem. 1987 Mar;101(3):591-9. doi: 10.1093/jb/101.3.591.

Abstract

A cDNA for porcine pancreatic elastase I has been cloned and used as a probe for blot hybridization analysis. Southern blot analysis of total DNA demonstrated that the elastase I gene is conserved among the porcine, rat, calf, and human genomes. Northern blot analysis, however, indicated that the elastase I gene is not expressed in the human adult pancreas, even though abundant expression is observed in the rat and porcine pancreas. Sequence analysis of the cloned human elastase I gene revealed that the proximal 5'-flanking region from -1 to -200 shows 78% identity with that of the actively transcribed rat elastase I gene, and contains a consensus TATA box and a putative tissue specific enhancer sequence. The transcriptional activity of the human elastase I gene appears to be suppressed in the human adult pancreas.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA / analysis
  • Humans
  • Nucleic Acid Hybridization
  • Pancreas / enzymology*
  • Pancreatic Elastase / genetics*
  • Rats
  • Species Specificity
  • Swine
  • Transcription, Genetic

Substances

  • DNA
  • Pancreatic Elastase

Associated data

  • GENBANK/X05293