Molecular insight into the PCNA-binding mode of FBH1

Structure. 2023 May 4;31(5):511-517.e3. doi: 10.1016/j.str.2023.03.004. Epub 2023 Mar 28.

Abstract

F-box DNA helicase 1 (FBH1) is involved in the regulation of cell responses to replicative stress. FBH1 is recruited to stalled DNA replication fork by PCNA where it inhibits homologous recombination and catalyzes fork regression. Here, we report the structural basis for the molecular recognition of two distinctly different motifs of FBH1, FBH1PIP and FBH1APIM, by PCNA. The crystal structure of PCNA in complex with FBH1PIP and analysis of NMR perturbations reveal overlapped FBH1PIP and FBH1APIM binding sites of PCNA and the dominant contribution of FBH1PIP in this interaction.

Keywords: APIM; FBH1; PCNA; PIP; mechanism.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • DNA Helicases* / metabolism
  • DNA Replication*
  • Homologous Recombination
  • Humans
  • Proliferating Cell Nuclear Antigen / genetics
  • Proliferating Cell Nuclear Antigen / metabolism

Substances

  • DNA Helicases
  • Proliferating Cell Nuclear Antigen
  • FBH1 protein, human