Characterization of RNA polymerase type II from human term placenta

J Cell Physiol. 1986 Jun;127(3):432-8. doi: 10.1002/jcp.1041270312.

Abstract

RNA polymerase type II from human term placenta has been isolated and characterized with respect to its template, ammonium sulfate, divalent cation, and buffer preferences. In addition, the apparent Michaelis constants for AMP and UMP incorporation have been determined. The enzyme was also analyzed by native and denaturing polyacrylamide gel electrophoresis, and evidence is presented that a single polypeptide is radiolabeled with azido purine nucleoside triphosphate photoprobes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Triphosphate / analogs & derivatives
  • Adenosine Triphosphate / metabolism
  • Azides / metabolism
  • Electrophoresis, Polyacrylamide Gel
  • Female
  • Guanosine Triphosphate / analogs & derivatives
  • Guanosine Triphosphate / metabolism
  • Humans
  • Kinetics
  • Macromolecular Substances
  • Molecular Weight
  • Placenta / enzymology*
  • Pregnancy
  • RNA Polymerase II / metabolism*
  • Uridine Triphosphate / metabolism

Substances

  • 8-azidoguanosine triphosphate
  • Azides
  • Macromolecular Substances
  • 8-azidoadenosine 5'-triphosphate
  • Guanosine Triphosphate
  • Adenosine Triphosphate
  • RNA Polymerase II
  • Uridine Triphosphate