Modified elastic tissue-Masson trichrome stain

Stain Technol. 1984 Jul;59(4):213-6. doi: 10.3109/10520298409113858.

Abstract

A combined elastic tissue-Masson technique is presented which stains elastic fibers of all sizes, nuclei and connective tissue. The modified elastic tissue stain consists of hematoxylin, ferric chloride and Verhoeff's iodine; nuclei and elastic fibers are stained blue-black in six minutes without differentiation. By contrast, cytoplasmic elements are stained red, (Biebrich scarlet-acid fuchsin) and collagen is stained green (light green) or blue (aniline blue). The entire staining procedure takes approximately one hour.

Publication types

  • Comparative Study

MeSH terms

  • Arteries / cytology
  • Chlorides
  • Elastic Tissue / cytology*
  • Ferric Compounds
  • Hematoxylin
  • Humans
  • Iodine
  • Lung / cytology
  • Myocardium / cytology
  • Staining and Labeling / methods*

Substances

  • Chlorides
  • Ferric Compounds
  • Iodine
  • ferric chloride
  • Hematoxylin