Abstract
NN'-Dicyclohexylcarbodi-imide at low concentrations decreases the H+/2e ratio for rat liver mitochondria over the span succinate to oxygen from 5.9 +/- 0.3 (mean +/- S.E.M.) to 4.0 +/- 0.1 and for the cytochrome b-c1 complex from 3.8 +/- 0.2 to 1.9 +/- 0.1, but has little effect on the H+/2e ratio of cytochrome oxidase. The decrease in stoicheiometry is due, not to uncoupling or inhibition of electron transport, but to inhibition of proton translocation. NN'-Dicyclohexylcarbodi-imide thus 'decouples' proton translocation in the cytochrome b-c1 complex.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Carbodiimides / pharmacology*
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Cell Membrane Permeability / drug effects
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Dicyclohexylcarbodiimide / pharmacology*
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Electron Transport Complex III
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In Vitro Techniques
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Mitochondria, Liver / drug effects
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Mitochondria, Liver / enzymology*
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Multienzyme Complexes / antagonists & inhibitors*
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NADH, NADPH Oxidoreductases / antagonists & inhibitors*
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Protons*
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Quinone Reductases / antagonists & inhibitors*
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Rats
Substances
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Carbodiimides
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Multienzyme Complexes
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Protons
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Dicyclohexylcarbodiimide
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NADH, NADPH Oxidoreductases
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Quinone Reductases
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Electron Transport Complex III