Isolation and characterization of a full-length expressible cDNA for human hypoxanthine phosphoribosyl transferase

Proc Natl Acad Sci U S A. 1983 Jan;80(2):477-81. doi: 10.1073/pnas.80.2.477.

Abstract

We have cloned a full-length 1.6-kilobase cDNA of a human mRNA coding for hypoxanthine phosphoribosyltransferase (HPRT; IMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.8) into a simian virus 40-based expression vector and have determined its full nucleotide sequence. The inferred amino acid sequence agrees with a partial amino acid sequence determined for authentic human HPRT protein. Transfection of HPRT-deficient mouse LA9 cells with the purified plasmid leads to the expression of human HPRT enzyme activity in cells stably transfected and selected for enzyme activity in hypoxanthine/aminopterin/thymidine medium.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular*
  • DNA / isolation & purification*
  • Humans
  • Hypoxanthine Phosphoribosyltransferase / deficiency
  • Hypoxanthine Phosphoribosyltransferase / genetics*
  • L Cells / enzymology
  • Mice
  • Plasmids
  • RNA, Messenger / genetics
  • Simian virus 40 / genetics
  • Transfection

Substances

  • RNA, Messenger
  • DNA
  • Hypoxanthine Phosphoribosyltransferase

Associated data

  • GENBANK/J00205