Abstract
We report the complete nucleotide sequence of a human beta actin cDNA. Both the 5' and 3' untranslated regions of the sequence are similar (greater than 80%) to the analogous regions of the rat beta-actin gene reported by Nudel et al (1983). When a segment of the 3' untranslated region is used as a radiolabelled probe, strong hybridization to chick beta actin mRNA is seen. This conservation of sequences suggests that strong selective pressures operate on non-translated segments of beta actin mRNA.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Actins / genetics*
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Amino Acid Sequence
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Animals
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Base Sequence
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Biological Evolution*
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Brain / metabolism
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Chickens
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Codon
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DNA / metabolism*
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DNA Restriction Enzymes
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Genes*
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Humans
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Liver / metabolism
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Muscles / metabolism
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Myocardium / metabolism
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Nucleic Acid Hybridization
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Protein Biosynthesis*
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RNA, Messenger / genetics*
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Rats
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Species Specificity
Substances
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Actins
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Codon
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RNA, Messenger
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DNA
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DNA Restriction Enzymes
Associated data
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GENBANK/J00074
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GENBANK/M10278
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GENBANK/X00351