Abstract
The complete sequence of the HindIII-BamHI/BglII fragment of plasmid pSV2 gpt, which contains the E. coli gpt gene, has been determined and used to predict the amino acid sequence of the protein. The nucleotide sequence was used to create a map of eight gpt gene deletion mutants which were generated in mammalian cells.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Bacterial Proteins / genetics*
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Base Sequence
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Chromosome Deletion
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Chromosomes, Bacterial / ultrastructure
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Cricetinae
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Cricetulus
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Escherichia coli / enzymology
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Escherichia coli / genetics*
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Genes
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Genes, Bacterial*
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Pentosyltransferases / genetics*
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Plasmids
Substances
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Bacterial Proteins
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Pentosyltransferases
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xanthine phosphoribosyltransferase