Abstract
The first complete sequence of the variable region of a kappa-light chain (V kappa) from a mouse anti-(streptococcal group A polysaccharide) antibody (immunoglobulin 7S34.1) is reported. Immunoglobulin 7S34.1 was isolated from the ascitic fluid of hybridoma 7S34.1 previously cloned in vitro. A newly developed technique for the isolation of peptides by using pre-column formation of peptide derivatives with dimethylaminoazobenzene isothiocyanate also served to complete the sequence. The sequence of the variable region of the kappa-light chain of immunoglobulin 7S34.1 defines a new mouse V kappa isotype (V kappa 27) and is the first mouse immunoglobulin light-chain variable region to be shown to have an extra cysteine residue at position 48.
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Bacterial*
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Antibodies, Monoclonal*
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Binding Sites, Antibody*
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Chromatography, High Pressure Liquid
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Hybridomas
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Immunoglobulin Light Chains*
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Immunoglobulin Variable Region*
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Immunoglobulin kappa-Chains*
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Isothiocyanates*
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Mice
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Peptide Fragments / analysis
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Polysaccharides, Bacterial / immunology*
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Streptococcus pyogenes / immunology*
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Thiocyanates
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p-Dimethylaminoazobenzene / analogs & derivatives
Substances
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Antibodies, Bacterial
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Antibodies, Monoclonal
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Immunoglobulin Light Chains
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Immunoglobulin Variable Region
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Immunoglobulin kappa-Chains
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Isothiocyanates
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Peptide Fragments
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Polysaccharides, Bacterial
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Thiocyanates
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streptococcal polysaccharide group A
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4-(N,N-dimethylaminoazobenzene)-4'-isothiocyanate
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p-Dimethylaminoazobenzene