The anti-oxidant activity of turmeric (Curcuma longa)

J Ethnopharmacol. 1995 Jul 7;47(2):59-67. doi: 10.1016/0378-8741(95)01250-h.

Abstract

The turmeric anti-oxidant protein (TAP) had been isolated from the aqueous extract of turmeric. The anti-oxidant principle was found to be a heat stable protein. Trypsin treatment abolished the anti-oxidant activity. The anti-oxidant principle had an absorbance maximum at 280 nm. After gel filtration, the protein showed a 2-fold increase in anti-oxidant activity and showed 2 bands in the SDS-PAGE with approximate molecular weight range of 24,000 Da. The protein showed a concentration-dependent inhibitory effect on the promoter induced lipid peroxidation. A 50% inhibitory activity of lipid peroxidation was observed at a protein concentration of 50 micrograms/ml. Ca(2+)-ATPase of rat brain homogenate was protected to nearly 50% of the initial activity from the lipid peroxidant induced inactivation by this protein. This protection of Ca(2+)-ATPase activity was found to be associated with the prevention of loss of -SH groups.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / chemistry
  • Antioxidants / pharmacology*
  • Brain / drug effects
  • Brain / enzymology
  • Calcium-Transporting ATPases / metabolism
  • Chromatography, Gel
  • Cod Liver Oil / metabolism
  • Curcumin / chemistry
  • Curcumin / pharmacology*
  • Dose-Response Relationship, Drug
  • Electrophoresis, Polyacrylamide Gel
  • Hot Temperature
  • Lipid Peroxidation / drug effects*
  • Male
  • Molecular Weight
  • Plant Oils / chemistry
  • Plant Oils / isolation & purification
  • Plant Oils / pharmacology*
  • Plant Proteins / chemistry
  • Plant Proteins / pharmacology*
  • Rats
  • Rats, Wistar
  • Spectrophotometry, Ultraviolet
  • Sulfhydryl Compounds / metabolism
  • Thiobarbituric Acid Reactive Substances / metabolism
  • Trypsin / metabolism
  • Trypsin / pharmacology

Substances

  • Antioxidants
  • Plant Oils
  • Plant Proteins
  • Sulfhydryl Compounds
  • Thiobarbituric Acid Reactive Substances
  • Cod Liver Oil
  • Trypsin
  • Calcium-Transporting ATPases
  • Curcumin