Analysis of pyrrolizidine alkaloids: a competitive enzyme-linked immunoassay (ELISA) for the quantitative determination of some toxic pyrrolizidine alkaloids

Nat Toxins. 1995;3(4):305-9; discussion 317. doi: 10.1002/nt.2620030426.

Abstract

A highly sensitive and specific competitive enzyme-linked immunoassay (ELISA) for the determination of retrorsine, senecionine, and integerrimine in the nanogram range has been developed. Pyrrolizidine alkaloids were detected in Senecio rupestris. No cross-reactivity was seen with either retrosine N-oxide, otonecine alkaloids, or other macrocyclic pyrrolizidine alkaloids.

MeSH terms

  • Animals
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Pyrrolizidine Alkaloids / analysis*
  • Rabbits
  • Reference Standards

Substances

  • Pyrrolizidine Alkaloids
  • retrorsine