Molecular characterization of the genes encoding the tungsten-containing aldehyde ferredoxin oxidoreductase from Pyrococcus furiosus and formaldehyde ferredoxin oxidoreductase from Thermococcus litoralis

J Bacteriol. 1995 Aug;177(16):4817-9. doi: 10.1128/jb.177.16.4817-4819.1995.

Abstract

The hyperthermophilic archaea Pyrococcus furiosus and Thermococcus litoralis contain the tungstoenzymes aldehyde ferredoxin oxidoreductase, a homodimer, and formaldehyde ferredoxin oxidoreductase, a homotetramer. herein we report the cloning and sequencing of the P. furiosus gene aor (605 residues; M(r), 66,630) and the T. litoralis gene for (621 residues; M(r), 68,941).

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aldehyde Oxidoreductases / classification
  • Aldehyde Oxidoreductases / genetics*
  • Amino Acid Sequence
  • Archaea / enzymology
  • Archaea / genetics*
  • Cloning, Molecular
  • Genes, Bacterial / genetics*
  • Metalloproteins / genetics*
  • Molecular Sequence Data
  • Restriction Mapping
  • Sequence Analysis
  • Sequence Homology, Amino Acid
  • Tungsten*

Substances

  • Metalloproteins
  • Aldehyde Oxidoreductases
  • aldehyde ferredoxin oxidoreductase
  • formaldehyde ferredoxin oxidoreductase
  • Tungsten

Associated data

  • GENBANK/X79777
  • GENBANK/X83963