Abstract
We investigated the potential of a rapid colorimetric microassay based on the reduction of dimethylthiazol-diphenyltetrazolium bromide (MTT) for determining the growth of Mycobacterium avium-M. intracellulare complex (MAC) and MICs of clofazimine, resorcinomycin A, and the quinolone PD 127391 against MAC. The reduction of MTT was directly proportional to the number of viable bacteria. A comparison of the MTT reduction test with the [3H]glycerol uptake assay showed the former to possess higher analytical sensitivity for detecting MAC growth in microtiter plates. The MIT reduction test avoids the use of radioisotopes and costly material and equipment; it is reliable, reproducible, and convenient for rapid routine susceptibility testing of MAC.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / administration & dosage
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Anti-Infective Agents / administration & dosage
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Biological Transport, Active
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Clofazimine / administration & dosage
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Colorimetry / methods*
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Colorimetry / statistics & numerical data
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Dipeptides / administration & dosage
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Fluoroquinolones*
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Glycerol / pharmacokinetics
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Humans
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Microbial Sensitivity Tests / methods*
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Microbial Sensitivity Tests / statistics & numerical data
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Mycobacterium avium Complex / drug effects*
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Mycobacterium avium Complex / growth & development
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Mycobacterium avium Complex / metabolism
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Oxidation-Reduction
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Quinolones / administration & dosage
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Radiometry / methods
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Radiometry / statistics & numerical data
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Sensitivity and Specificity
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Tetrazolium Salts / metabolism
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Thiazoles / metabolism
Substances
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Anti-Bacterial Agents
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Anti-Infective Agents
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Dipeptides
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Fluoroquinolones
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Quinolones
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Tetrazolium Salts
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Thiazoles
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resorcinomycin A
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clinafloxacin
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Clofazimine
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thiazolyl blue
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Glycerol