Abstract
The type 1 ribosome-inactivating protein crotin 2 depurinated Escherichia coli ribosomes which, upon treatment of the isolated rRNA with acid aniline, released a fragment of around 240 nucleotides whose 5'-end sequence was 5'-GAGGACCGGAGUGGAC-3'. The formation of fusidic acid-dependent ribosomal complexes completely prevented release of the fragment. Ribosomes from crotin 2-pretreated fusidic acid complexes were insensitive to acid aniline. They released the RNA fragment only after a second treatment with crotin 2 and acid aniline whereas unprotected ribosomes released the fragment directly after acid aniline.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Apurinic Acid / metabolism
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Base Sequence
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Escherichia coli
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Fusidic Acid / pharmacology*
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Molecular Sequence Data
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N-Glycosyl Hydrolases / metabolism*
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Plant Proteins / pharmacology*
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Protein Biosynthesis / drug effects
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Protein Synthesis Inhibitors / pharmacology*
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RNA, Bacterial / chemistry
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RNA, Fungal / chemistry
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RNA, Ribosomal / chemistry*
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RNA, Ribosomal / drug effects
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Ribosome Inactivating Proteins
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Ribosome Inactivating Proteins, Type 1
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Ribosomes / drug effects*
Substances
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Plant Proteins
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Protein Synthesis Inhibitors
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RNA, Bacterial
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RNA, Fungal
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RNA, Ribosomal
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Ribosome Inactivating Proteins, Type 1
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Apurinic Acid
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Fusidic Acid
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N-Glycosyl Hydrolases
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Ribosome Inactivating Proteins