Abstract
cDNA clones encoding the low molecular weight GTP-binding proteins ral A (951 bp) and ral B (2073 bp), including the entire coding region (618 bp), were isolated from a rat PC12 pheochromocytoma library. Northern analyses demonstrated that both ral A and ral B are widely expressed in rat tissues. Two ral A transcripts of 1.1 and 2.9 kb were observed in most tissues in varying proportions. The 1.1 kb ral A band of testes was further shown to be composed of two closely migrating species. In contrast to these findings, a single ral B transcript of 2.3 kb was detected in most tissues. Steady-state levels of ral A transcripts appear greater than ral B. Quantitatively, the testes exhibited the highest ral A and ral B mRNA levels, with lower levels observed in the brain, adrenal and pituitary glands, kidney and ovary. Ral mRNA levels were lowest in muscle tissue, particularly skeletal muscle.
Publication types
-
Comparative Study
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Base Sequence
-
Blotting, Northern
-
Cloning, Molecular
-
DNA / isolation & purification
-
GTP-Binding Proteins / biosynthesis*
-
GTP-Binding Proteins / genetics*
-
Gene Expression*
-
Gene Library
-
Humans
-
Molecular Sequence Data
-
PC12 Cells
-
Poly A / genetics
-
Poly A / isolation & purification
-
Polymerase Chain Reaction
-
RNA / genetics
-
RNA / isolation & purification
-
RNA, Messenger / isolation & purification
-
RNA, Messenger / metabolism
-
Rats
-
Restriction Mapping
-
Sequence Homology, Amino Acid
-
Transcription, Genetic
-
ral GTP-Binding Proteins
Substances
-
RNA, Messenger
-
Ralb protein, rat
-
Poly A
-
RNA
-
DNA
-
GTP-Binding Proteins
-
ral GTP-Binding Proteins
Associated data
-
GENBANK/L16877
-
GENBANK/L16878
-
GENBANK/L19106
-
GENBANK/L19107
-
GENBANK/L19108
-
GENBANK/L19109
-
GENBANK/L19111
-
GENBANK/L19112
-
GENBANK/L19698
-
GENBANK/L19699